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KMID : 0382320060260010020
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Environmental Mutagens and Carcinogens
2006 Volume.26 No. 1 p.20 ~ p.24
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Isolation and Purification of Antimicrobial Substance from the Giant Snail, Achatina fulica
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Kim In-Hae
Lee Jae-Hwa
Hyun Jin-Won
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Abstract
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An antimicrobial substance was purified from the giant snail body extract (Achatina fulica) using solid-phase extraction and separation on HPLC reversed-phase chromatography. The primary structure were determined by a combination of an automated amino acid sequence and MALDI-TOF Mass. Its molecular mass was found to be 1392.64 Da. This result was in excellent agrement with the theoretical molecular mass calculated from the amino acid sequence. purified peptide showed antimicrobial activity in vitro against Escherichia coli D31. This result indicate that giant snail whole body was potentially antimicrobial.
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KEYWORD
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MALDI-TOF Mass, antimicrobial activity, giant snail
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